A new twist on PIFE: photoisomerisation-related fluorescence enhancement

no code implementations • 24 Feb 2023 • Evelyn Ploetz, Benjamin Ambrose, Anders Barth, Richard Börner, Felix Erichson, Achillefs N. Kapanidis, Harold D. Kim, Marcia Levitus, Timothy M. Lohman, Abhishek Mazumder, David S. Rueda, Fabio D. Steffen, Thorben Cordes, Steven W. Magennis, Eitan Lerner

PIFE was first used as an acronym for protein-induced fluorescence enhancement, which refers to the increase in fluorescence observed upon the interaction of a fluorophore, such as a cyanine, with a protein.

The FRET-based structural dynamics challenge -- community contributions to consistent and open science practices

no code implementations • 4 Jun 2020 • Eitan Lerner, Benjamin Ambrose, Anders Barth, Victoria Birkedal, Scott C. Blanchard, Richard Borner, Thorben Cordes, Timothy D. Craggs, Taekjip Ha, Gilad Haran, Thorsten Hugel, Antonino Ingargiola, Achillefs Kapanidis, Don C. Lamb, Ted Laurence, Nam ki Lee, Edward A. Lemke, Emmanuel Margeat, Jens Michaelis, Xavier Michalet, Daniel Nettels, Thomas-Otavio Peulen, Benjamin Schuler, Claus A. M. Seidel, Hamid So-leimaninejad, Shimon Weiss

These recent efforts include multi-lab blind-tests to assess the accuracy and precision of smFRET between different labs using different procedures, the formal assembly of the FRET community and development of smFRET procedures to be considered for entries in the wwPDB.

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