Spatially variant PSF modeling in confocal macroscopy
Point spread function (PSF) plays an essential role in image reconstruction. In the context of confocal microscopy, optical performance degrades towards the edge of the field of view as astigmatism, coma and vignetting. Thus, one should expect the related artifacts to be even stronger in macroscopy, where the field of view is much larger. The field aberrations in macroscopy fluorescence imaging system was observed to be symmetrical and to increase with the distance from the center of the field of view. In this paper we propose an experiment and an optimization method for assessing the center of the field of view. The obtained results constitute a step towards reducing the number of parameters in macroscopy PSF model.
PDF AbstractTasks
Datasets
Add Datasets
introduced or used in this paper
Results from the Paper
Submit
results from this paper
to get state-of-the-art GitHub badges and help the
community compare results to other papers.
Methods
No methods listed for this paper. Add
relevant methods here
