Three-dimensional virtual refocusing of fluorescence microscopy images using deep learning

31 Jan 2019Yichen WuYair RivensonHongda WangYilin LuoEyal Ben-DavidLaurent A. BentolilaChristian PritzAydogan Ozcan

Three-dimensional (3D) fluorescence microscopy in general requires axial scanning to capture images of a sample at different planes. Here we demonstrate that a deep convolutional neural network can be trained to virtually refocus a 2D fluorescence image onto user-defined 3D surfaces within the sample volume... (read more)

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