Search Results for author: Alex L. Payne-Dwyer

Found 3 papers, 0 papers with code

RecA and RecB: probing complexes of DNA repair proteins with mitomycin C in live Escherichia coli with single-molecule sensitivity

no code implementations4 Oct 2021 Alex L. Payne-Dwyer, Aisha H. Syeda, Jack W. Shepherd, Lewis Frame, Mark. C. Leake

The RecA protein and RecBCD complex are key bacterial components for the maintenance and repair of DNA, RecBCD a helicase-nuclease that uses homologous recombination to resolve double-stranded DNA breaks and also facilitating decoration of single-stranded DNA with RecA to form RecA filaments, a vital step in the double-stranded break DNA repair pathway.

Single-molecular quantification of flowering control proteins within nuclear condensates in live whole Arabidopsis root

no code implementations31 Aug 2021 Alex L. Payne-Dwyer, Mark C. Leake

Here we describe the coupled standardisation of two complementary fluorescence imaging techniques and apply it to liquid-liquid phase separated condensates formed from an EGFP fluorescent reporter of Flowering Control Locus A (FCA), a protein that associates with chromosomal DNA in plants during epigenetic regulation of the flowering process.

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