no code implementations • 4 Oct 2021 • Alex L. Payne-Dwyer, Aisha H. Syeda, Jack W. Shepherd, Lewis Frame, Mark. C. Leake
The RecA protein and RecBCD complex are key bacterial components for the maintenance and repair of DNA, RecBCD a helicase-nuclease that uses homologous recombination to resolve double-stranded DNA breaks and also facilitating decoration of single-stranded DNA with RecA to form RecA filaments, a vital step in the double-stranded break DNA repair pathway.
no code implementations • 31 Aug 2021 • Alex L. Payne-Dwyer, Mark C. Leake
Here we describe the coupled standardisation of two complementary fluorescence imaging techniques and apply it to liquid-liquid phase separated condensates formed from an EGFP fluorescent reporter of Flowering Control Locus A (FCA), a protein that associates with chromosomal DNA in plants during epigenetic regulation of the flowering process.
no code implementations • 24 Jun 2021 • Xin Jin, Ji-Eun Lee, Charley Schaefer, Xinwei Luo, Adam J. M. Wollman, Alex L. Payne-Dwyer, Tian Tian, Xiaowei Zhang, Xiao Chen, Yingxing Li, Tom C. B. McLeish, Mark C. Leake, Fan Bai
Liquid-liquid phase separation is emerging as a crucial phenomenon in several fundamental cell processes.
