no code implementations • 10 Aug 2024 • Guangdong Ma, Che-Yung Shen, Jingxi Li, Luzhe Huang, Cagatay Isil, Fazil Onuralp Ardic, Xilin Yang, Yuhang Li, Yuntian Wang, Md Sadman Sakib Rahman, Aydogan Ozcan
Here, we report unidirectional imaging under spatially partially coherent light and demonstrate high-quality imaging only in the forward direction (A->B) with high power efficiency while distorting the image formation in the backward direction (B->A) along with low power efficiency.
no code implementations • 7 Jul 2024 • Luzhe Huang, Xiongye Xiao, Shixuan Li, Jiawen Sun, Yi Huang, Aydogan Ozcan, Paul Bogdan
The advance of diffusion-based generative models in recent years has revolutionized state-of-the-art (SOTA) techniques in a wide variety of image analysis and synthesis tasks, whereas their adaptation on image restoration, particularly within computational microscopy remains theoretically and empirically underexplored.
no code implementations • 29 Apr 2024 • Luzhe Huang, Yuzhu Li, Nir Pillar, Tal Keidar Haran, William Dean Wallace, Aydogan Ozcan
Here, we present an autonomous quality and hallucination assessment method (termed AQuA), mainly designed for virtual tissue staining, while also being applicable to histochemical staining.
no code implementations • 21 Mar 2024 • Michael John Fanous, Paloma Casteleiro Costa, Cagatay Isil, Luzhe Huang, Aydogan Ozcan
The integration of deep learning techniques with biophotonic setups has opened new horizons in bioimaging.
no code implementations • 2 Aug 2023 • Yuzhu Li, Nir Pillar, Jingxi Li, Tairan Liu, Di wu, Songyu Sun, Guangdong Ma, Kevin De Haan, Luzhe Huang, Sepehr Hamidi, Anatoly Urisman, Tal Keidar Haran, William Dean Wallace, Jonathan E. Zuckerman, Aydogan Ozcan
Histological examination is a crucial step in an autopsy; however, the traditional histochemical staining of post-mortem samples faces multiple challenges, including the inferior staining quality due to autolysis caused by delayed fixation of cadaver tissue, as well as the resource-intensive nature of chemical staining procedures covering large tissue areas, which demand substantial labor, cost, and time.
no code implementations • 22 May 2023 • Luzhe Huang, Jianing Li, Xiaofu Ding, Yijie Zhang, Hanlong Chen, Aydogan Ozcan
Uncertainty estimation is critical for numerous applications of deep neural networks and draws growing attention from researchers.
no code implementations • 9 Jan 2023 • Hanlong Chen, Luzhe Huang, Tairan Liu, Aydogan Ozcan
The application of deep learning techniques has greatly enhanced holographic imaging capabilities, leading to improved phase recovery and image reconstruction.
no code implementations • 17 Sep 2022 • Luzhe Huang, Hanlong Chen, Tairan Liu, Aydogan Ozcan
Here, we report a self-supervised learning model, termed GedankenNet, that eliminates the need for labeled or experimental training data, and demonstrate its effectiveness and superior generalization on hologram reconstruction tasks.
no code implementations • 6 Jul 2022 • Yijie Zhang, Luzhe Huang, Tairan Liu, Keyi Cheng, Kevin De Haan, Yuzhu Li, Bijie Bai, Aydogan Ozcan
Here, we introduce a fast virtual staining framework that can stain defocused autofluorescence images of unlabeled tissue, achieving equivalent performance to virtual staining of in-focus label-free images, also saving significant imaging time by lowering the microscope's autofocusing precision.
no code implementations • 22 Apr 2022 • Hanlong Chen, Luzhe Huang, Tairan Liu, Aydogan Ozcan
Deep learning-based image reconstruction methods have achieved remarkable success in phase recovery and holographic imaging.
no code implementations • 27 Jan 2022 • Luzhe Huang, Xilin Yang, Tairan Liu, Aydogan Ozcan
Here, we demonstrate a few-shot transfer learning method that helps a holographic image reconstruction deep neural network rapidly generalize to new types of samples using small datasets.
no code implementations • 12 Feb 2021 • Luzhe Huang, Tairan Liu, Xilin Yang, Yi Luo, Yair Rivenson, Aydogan Ozcan
Digital holography is one of the most widely used label-free microscopy techniques in biomedical imaging.
no code implementations • 22 Dec 2020 • Xilin Yang, Luzhe Huang, Yilin Luo, Yichen Wu, Hongda Wang, Yair Rivenson, Aydogan Ozcan
We present a virtual image refocusing method over an extended depth of field (DOF) enabled by cascaded neural networks and a double-helix point-spread function (DH-PSF).
no code implementations • 21 Oct 2020 • Luzhe Huang, Yilin Luo, Yair Rivenson, Aydogan Ozcan
Volumetric imaging of samples using fluorescence microscopy plays an important role in various fields including physical, medical and life sciences.
no code implementations • 21 Mar 2020 • Yilin Luo, Luzhe Huang, Yair Rivenson, Aydogan Ozcan
We demonstrate a deep learning-based offline autofocusing method, termed Deep-R, that is trained to rapidly and blindly autofocus a single-shot microscopy image of a specimen that is acquired at an arbitrary out-of-focus plane.