Virtual Gram staining of label-free bacteria using darkfield microscopy and deep learning

no code implementations • 17 Jul 2024 • Cagatay Isil, Hatice Ceylan Koydemir, Merve Eryilmaz, Kevin De Haan, Nir Pillar, Koray Mentesoglu, Aras Firat Unal, Yair Rivenson, Sukantha Chandrasekaran, Omai B. Garner, Aydogan Ozcan

Gram staining has been one of the most frequently used staining protocols in microbiology for over a century, utilized across various fields, including diagnostics, food safety, and environmental monitoring.

Rapid and stain-free quantification of viral plaque via lens-free holography and deep learning

no code implementations • 30 Jun 2022 • Tairan Liu, Yuzhu Li, Hatice Ceylan Koydemir, Yijie Zhang, Ethan Yang, Merve Eryilmaz, Hongda Wang, Jingxi Li, Bijie Bai, Guangdong Ma, Aydogan Ozcan

We also demonstrated that this data-driven plaque assay offers the capability of quantifying the infected area of the cell monolayer, performing automated counting and quantification of PFUs and virus-infected areas over a 10-fold larger dynamic range of virus concentration than standard viral plaque assays.

Specificity Virology