no code implementations • 5 Jun 2023 • Kevin C. Zhou, Mark Harfouche, Maxwell Zheng, Joakim Jönsson, Kyung Chul Lee, Ron Appel, Paul Reamey, Thomas Doman, Veton Saliu, Gregor Horstmeyer, Roarke Horstmeyer
We present a large-scale computational 3D topographic microscope that enables 6-gigapixel profilometric 3D imaging at micron-scale resolution across $>$110 cm$^2$ areas over multi-millimeter axial ranges.
no code implementations • 14 Mar 2023 • Lucas Kreiss, Shaowei Jiang, Xiang Li, Shiqi Xu, Kevin C. Zhou, Alexander Mühlberg, Kyung Chul Lee, Kanghyun Kim, Amey Chaware, Michael Ando, Laura Barisoni, Seung Ah Lee, Guoan Zheng, Kyle Lafata, Oliver Friedrich, Roarke Horstmeyer
Until recently, conventional biochemical staining had the undisputed status as well-established benchmark for most biomedical problems related to clinical diagnostics, fundamental research and biotechnology.
Benefiting from its single-photon sensitivity, single-photon avalanche diode (SPAD) array has been widely applied in various fields such as fluorescence lifetime imaging and quantum computing.
no code implementations • 30 Nov 2022 • Mark Harfouche, Kanghyun Kim, Kevin C. Zhou, Pavan Chandra Konda, Sunanda Sharma, Eric E. Thomson, Colin Cooke, Shiqi Xu, Lucas Kreiss, Amey Chaware, Xi Yang, Xing Yao, Vinayak Pathak, Martin Bohlen, Ron Appel, Aurélien Bègue, Clare Cook, Jed Doman, John Efromson, Gregor Horstmeyer, Jaehee Park, Paul Reamey, Veton Saliu, Eva Naumann, Roarke Horstmeyer
This article experimentally examines different configurations of a novel multi-camera array microscope (MCAM) imaging technology.
We report Tensorial Tomographic Differential Phase-Contrast microscopy (T2DPC), a quantitative label-free tomographic imaging method for simultaneous measurement of phase and anisotropy.
no code implementations • 4 Apr 2022 • Shiqi Xu, Wenhui Liu, Xi Yang, Joakim Jönsson, Ruobing Qian, Paul McKee, Kanghyun Kim, Pavan Chandra Konda, Kevin C. Zhou, Lucas Kreiß, Haoqian Wang, Edouard Berrocal, Scott Huettel, Roarke Horstmeyer
We evaluate our setup by classifying different spatiotemporal-decorrelating patterns hidden beneath a 5mm tissue-like phantom made with rapidly decorrelating dynamic scattering media.
no code implementations • 10 Oct 2021 • Xing Yao, Vinayak Pathak, Haoran Xi, Amey Chaware, Colin Cooke, Kanghyun Kim, Shiqi Xu, Yuting Li, Timothy Dunn, Pavan Chandra Konda, Kevin C. Zhou, Roarke Horstmeyer
This work demonstrates a multi-lens microscopic imaging system that overlaps multiple independent fields of view on a single sensor for high-efficiency automated specimen analysis.
no code implementations • 3 Jul 2021 • Shiqi Xu, Xi Yang, Wenhui Liu, Joakim Jonsson, Ruobing Qian, Pavan Chandra Konda, Kevin C. Zhou, Lucas Kreiss, Qionghai Dai, Haoqian Wang, Edouard Berrocal, Roarke Horstmeyer
Noninvasive optical imaging through dynamic scattering media has numerous important biomedical applications but still remains a challenging task.
We present a feature-free photogrammetric technique that enables quantitative 3D mesoscopic (mm-scale height variation) imaging with tens-of-micron accuracy from sequences of images acquired by a smartphone at close range (several cm) under freehand motion without additional hardware.
no code implementations • 15 Oct 2020 • Tri Vu, Anthony DiSpirito III, Daiwei Li, Zixuan Zhang, Xiaoyi Zhu, Maomao Chen, Laiming Jiang, Dong Zhang, Jianwen Luo, Yu Shrike Zhang, Qifa Zhou, Roarke Horstmeyer, Junjie Yao
Photoacoustic microscopy (PAM) is an emerging imaging method combining light and sound.
Standard microscopes offer a variety of settings to help improve the visibility of different specimens to the end microscope user.
One primary technical challenge in photoacoustic microscopy (PAM) is the necessary compromise between spatial resolution and imaging speed.
This paper introduces a new method of data-driven microscope design for virtual fluorescence microscopy.
We present a tomographic imaging technique, termed Deep Prior Diffraction Tomography (DP-DT), to reconstruct the 3D refractive index (RI) of thick biological samples at high resolution from a sequence of low-resolution images collected under angularly varying illumination.
Image and Video Processing Biological Physics Optics
Here we focus on one such degree of freedom - illumination within a microscope - which can drastically alter information captured by the image sensor.
Recent advances in ptychography have demonstrated that one can image beyond the diffraction limit of the objective lens in a microscope.