no code implementations • 2 Aug 2023 • Yuzhu Li, Nir Pillar, Jingxi Li, Tairan Liu, Di wu, Songyu Sun, Guangdong Ma, Kevin De Haan, Luzhe Huang, Sepehr Hamidi, Anatoly Urisman, Tal Keidar Haran, William Dean Wallace, Jonathan E. Zuckerman, Aydogan Ozcan
Histological examination is a crucial step in an autopsy; however, the traditional histochemical staining of post-mortem samples faces multiple challenges, including the inferior staining quality due to autolysis caused by delayed fixation of cadaver tissue, as well as the resource-intensive nature of chemical staining procedures covering large tissue areas, which demand substantial labor, cost, and time.
no code implementations • 9 Jan 2023 • Hanlong Chen, Luzhe Huang, Tairan Liu, Aydogan Ozcan
The application of deep learning techniques has greatly enhanced holographic imaging capabilities, leading to improved phase recovery and image reconstruction.
no code implementations • 17 Sep 2022 • Luzhe Huang, Hanlong Chen, Tairan Liu, Aydogan Ozcan
Here, we report a self-supervised learning model, termed GedankenNet, that eliminates the need for labeled or experimental training data, and demonstrate its effectiveness and superior generalization on hologram reconstruction tasks.
no code implementations • 30 Aug 2022 • Yi Luo, Yijie Zhang, Tairan Liu, Alan Yu, Yichen Wu, Aydogan Ozcan
To address this need, we present a mobile and cost-effective label-free bio-aerosol sensor that takes holographic images of flowing particulate matter concentrated by a virtual impactor, which selectively slows down and guides particles larger than ~6 microns to fly through an imaging window.
no code implementations • 14 Jul 2022 • Xilin Yang, Bijie Bai, Yijie Zhang, Yuzhu Li, Kevin De Haan, Tairan Liu, Aydogan Ozcan
Unlike a single neural network structure which only takes one stain type as input to digitally output images of another stain type, C-DNN first uses virtual staining to transform autofluorescence microscopy images into H&E and then performs stain transfer from H&E to the domain of the other stain in a cascaded manner.
no code implementations • 6 Jul 2022 • Yijie Zhang, Luzhe Huang, Tairan Liu, Keyi Cheng, Kevin De Haan, Yuzhu Li, Bijie Bai, Aydogan Ozcan
Here, we introduce a fast virtual staining framework that can stain defocused autofluorescence images of unlabeled tissue, achieving equivalent performance to virtual staining of in-focus label-free images, also saving significant imaging time by lowering the microscope's autofocusing precision.
no code implementations • 30 Jun 2022 • Tairan Liu, Yuzhu Li, Hatice Ceylan Koydemir, Yijie Zhang, Ethan Yang, Merve Eryilmaz, Hongda Wang, Jingxi Li, Bijie Bai, Guangdong Ma, Aydogan Ozcan
We also demonstrated that this data-driven plaque assay offers the capability of quantifying the infected area of the cell monolayer, performing automated counting and quantification of PFUs and virus-infected areas over a 10-fold larger dynamic range of virus concentration than standard viral plaque assays.
no code implementations • 7 May 2022 • Yuzhu Li, Tairan Liu, Hatice Ceylan Koydemir, Hongda Wang, Keelan O'Riordan, Bijie Bai, Yuta Haga, Junji Kobashi, Hitoshi Tanaka, Takaya Tamaru, Kazunori Yamaguchi, Aydogan Ozcan
Due to the large scalability, ultra-large field-of-view, and low cost of the TFT-based image sensors, this platform can be integrated with each agar plate to be tested and disposed of after the automated CFU count.
no code implementations • 22 Apr 2022 • Hanlong Chen, Luzhe Huang, Tairan Liu, Aydogan Ozcan
Deep learning-based image reconstruction methods have achieved remarkable success in phase recovery and holographic imaging.
no code implementations • 27 Jan 2022 • Luzhe Huang, Xilin Yang, Tairan Liu, Aydogan Ozcan
Here, we demonstrate a few-shot transfer learning method that helps a holographic image reconstruction deep neural network rapidly generalize to new types of samples using small datasets.
no code implementations • 4 Mar 2021 • Yijie Zhang, Tairan Liu, Manmohan Singh, Yilin Luo, Yair Rivenson, Kirill V. Larin, Aydogan Ozcan
Using 2-fold undersampled spectral data (i. e., 640 spectral points per A-line), the trained neural network can blindly reconstruct 512 A-lines in ~6. 73 ms using a desktop computer, removing spatial aliasing artifacts due to spectral undersampling, also presenting a very good match to the images of the same samples, reconstructed using the full spectral OCT data (i. e., 1280 spectral points per A-line).
no code implementations • 12 Feb 2021 • Luzhe Huang, Tairan Liu, Xilin Yang, Yi Luo, Yair Rivenson, Aydogan Ozcan
Digital holography is one of the most widely used label-free microscopy techniques in biomedical imaging.
no code implementations • 20 Aug 2020 • Kevin de Haan, Yijie Zhang, Jonathan E. Zuckerman, Tairan Liu, Anthony E. Sisk, Miguel F. P. Diaz, Kuang-Yu Jen, Alexander Nobori, Sofia Liou, Sarah Zhang, Rana Riahi, Yair Rivenson, W. Dean Wallace, Aydogan Ozcan
Based on evaluation by three renal pathologists, followed by adjudication by a fourth renal pathologist, we show that the generation of virtual special stains from existing H&E images improves the diagnosis in several non-neoplastic kidney diseases sampled from 58 unique subjects.
no code implementations • 1 Jul 2020 • Tairan Liu, Kevin de Haan, Bijie Bai, Yair Rivenson, Yi Luo, Hongda Wang, David Karalli, Hongxiang Fu, Yibo Zhang, John FitzGerald, Aydogan Ozcan
Our analysis shows that a trained deep neural network can extract the birefringence information using both the sample specific morphological features as well as the holographic amplitude and phase distribution.
no code implementations • 15 Jul 2019 • Tairan Liu, Zhensong Wei, Yair Rivenson, Kevin De Haan, Yibo Zhang, Yichen Wu, Aydogan Ozcan
We report a framework based on a generative adversarial network (GAN) that performs high-fidelity color image reconstruction using a single hologram of a sample that is illuminated simultaneously by light at three different wavelengths.
no code implementations • 15 Oct 2018 • Tairan Liu, Kevin De Haan, Yair Rivenson, Zhensong Wei, Xin Zeng, Yibo Zhang, Aydogan Ozcan
We present a deep learning framework based on a generative adversarial network (GAN) to perform super-resolution in coherent imaging systems.
no code implementations • 20 Jul 2018 • Yair Rivenson, Tairan Liu, Zhensong Wei, Yibo Zhang, Aydogan Ozcan
Using a deep neural network, we demonstrate a digital staining technique, which we term PhaseStain, to transform quantitative phase images (QPI) of labelfree tissue sections into images that are equivalent to brightfield microscopy images of the same samples that are histochemically stained.