The LIVECell (Label-free In Vitro image Examples of Cells) dataset is a large-scale microscopic image dataset for instance-segmentation of individual cells in 2D cell cultures.

LIVECell consists of 5,239 manually annotated, expert-validated, Incucyte HD phase-contrast microscopy images with a total of 1,686,352 individual cells annotated from eight different cell types (average 313 cells per image). The LIVECell images have predefined splits into training (3188), validation (539) and test (1512) sets. Each split is also further subdivided into each of the eight cell types. The training set also has splits of different sizes (2, 4, 5, 25, 50%) to allow dataset size experimentation.