Joint Angular Refinement and Reconstruction for Single-Particle Cryo-EM

23 Mar 2020 Zehni Mona Donati Laurène Soubies Emmanuel Zhao Zhizhen J. Unser Michael

Single-particle cryo-electron microscopy (cryo-EM) reconstructs the three-dimensional (3D) structure of bio-molecules from a large set of 2D projection images with random and unknown orientations. A crucial step in the single-particle cryo-EM pipeline is 3D refinement, which resolves a high-resolution 3D structure from an initial approximate volume by refining the estimation of the orientation of each projection... (read more)

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