Combining oligo pools and Golden Gate cloning to create protein variant libraries or guide RNA libraries for CRISPR applications

no code implementations • 22 Jan 2024 • Alicia Maciá Valero, Rianne C. Prins, Thijs de Vroet, Sonja Billerbeck

Oligo pools are array-synthesized, user-defined mixtures of single-stranded oligonucleotides that can be used as a source of synthetic DNA for library cloning.

Small antimicrobial resistance proteins (SARPs): Small proteins conferring antimicrobial resistance

no code implementations • 27 Oct 2023 • Rianne C. Prins, Sonja Billerbeck

Small open reading frames are understudied as they have been historically excluded from genome annotations.

Improving homology-directed repair by small molecule agents for genetic engineering in unconventional yeast? -- Learning from the engineering of mammalian systems

no code implementations • 29 Aug 2023 • Min Lu, Sonja Billerbeck

Here, we consolidate knowledge of available small molecules that have been used to improve HR efficiency in mammalian cells and the few ones that have been used in yeast.

Functional Synthetic Biology

no code implementations • 1 Jul 2022 • Ibrahim Aldulijan, Jacob Beal, Sonja Billerbeck, Jeff Bouffard, Gael Chambonnier, Nikolaos Delkis, Isaac Guerreiro, Martin Holub, Paul Ross, Vinoo Selvarajah, Noah Sprent, Gonzalo Vidal, Alejandro Vignoni

Synthetic biologists have made great progress over the past decade in developing methods for modular assembly of genetic sequences and in engineering biological systems with a wide variety of functions in various contexts and organisms.