no code implementations • 21 Mar 2022 • Tasneem Jivanjee, Samira Ibrahim, Sarah K. Nyquist, G. James Gatter, Joshua D. Bromley, Swati Jaiswal, Bonnie Berger, Samuel M. Behar, J. Christopher Love, Alex K. Shalek
In short, a fraction of the 3' barcoded whole transcriptome amplification (WTA) product typically used to generate a massively parallel 3' scRNA-seq library is enriched for TCR transcripts using biotinylated probes, and further amplified using the same universal primer sequence from WTA.