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The End Restraint Method for Mechanically Perturbing Nucleic Acids in silico
While the broad-scale mechanical response of nucleic acids to tension and torsion is well characterized, detail at the single base pair level is beyond the limits of even super-resolution imaging.
Investigating molecular crowding during cell division in budding yeast with FRET
Cell division, aging, and stress recovery triggers spatial reorganization of cellular components in the cytoplasm, including membrane bound organelles, with molecular changes in their compositions and structures.
Correlative approaches in single-molecule biophysics: a review of the progress in methods and applications
Many new correlative single-molecule biophysics techniques also include more complex, physiologically relevant approaches as well as increasing number that combine advanced computational methods and mathematical analysis with experimental tools.
PySTACHIO: Python Single-molecule TrAcking stoiCHiometry Intensity and simulatiOn, a flexible, extensible, beginner-friendly and optimized program for analysis of single-molecule microscopy
As camera pixel arrays have grown larger and faster, and optical microscopy techniques ever more refined, there has been an explosion in the quantity of data acquired during routine light microcopy.
Localization microscopy: a review of the progress in methods and applications
Here, we report analysis and summary of research in the field of localization microscopy for optical imaging.
Super-Resolution
Optics
Biological Physics
Subcellular Processes
Molecular crowding in single eukaryotic cells: using cell environment biosensing and single-molecule optical microscopy to probe dependence on extracellular ionic strength, local glucose conditions, and sensor copy number
The physical and chemical environment inside cells is of fundamental importance to all life but has traditionally been difficult to determine on a subcellular basis.
