Virtual birefringence imaging and histological staining of amyloid deposits in label-free tissue using autofluorescence microscopy and deep learning

no code implementations • 14 Mar 2024 • Xilin Yang, Bijie Bai, Yijie Zhang, Musa Aydin, Sahan Yoruc Selcuk, Zhen Guo, Gregory A. Fishbein, Karine Atlan, William Dean Wallace, Nir Pillar, Aydogan Ozcan

Systemic amyloidosis is a group of diseases characterized by the deposition of misfolded proteins in various organs and tissues, leading to progressive organ dysfunction and failure.

Virtual histological staining of unlabeled autopsy tissue

no code implementations • 2 Aug 2023 • Yuzhu Li, Nir Pillar, Jingxi Li, Tairan Liu, Di wu, Songyu Sun, Guangdong Ma, Kevin De Haan, Luzhe Huang, Sepehr Hamidi, Anatoly Urisman, Tal Keidar Haran, William Dean Wallace, Jonathan E. Zuckerman, Aydogan Ozcan

Histological examination is a crucial step in an autopsy; however, the traditional histochemical staining of post-mortem samples faces multiple challenges, including the inferior staining quality due to autolysis caused by delayed fixation of cadaver tissue, as well as the resource-intensive nature of chemical staining procedures covering large tissue areas, which demand substantial labor, cost, and time.

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